The Cell Line Nucleofector™ Kit V is one of our five Nucleofector™ Kits suited for transfecting cell lines in the Nucleofector™ 2b Device. This kit has been determined to be optimal for transfecting following cell types: 32D 3T3-L1 pre-ad A20 A375 A7r5 AGS ARPE-19 B16-F10 BA/F3 (DSMZ) C2C12 C6 Capan-1 CHO [suspension] CHO-S [suspension] (Life Technologies) COS-1 HaCaT HCT116 HEK293 HepG2 HL-60 HUV-EC-C Jurkat K-562 (DSMZ) MCF7 MDA-MB-231 MDA-MB-468 NB-4 (DSMZ) NCTC clone 929 Neuro-2a [N2a] NG108-15 P19 PC-12 PC-3 Raji Ramos RAW 264.7 RBL-1 S49 Saos-2 Schneider‘s Drosophila Line 2 SH-SY5Y SK-N-SH SK-OV-3 SW480 T/C-28 a2 T/G HA-VSMC T-47D THP-1 U-2 OS Vero The respective cell type-specific Optimized Protocols are available in our knowledge database. In these Optimized Protocols the best Nucleofection conditions are indicated. In addition, we share our experience and knowledge for trea™ent of individual cell types. The kit is available in different variants for either 10 or 25 or 4 x 25 reactions.
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