Checking Sample Quality with the FlashGel™ System for RNA – Sample degradation is visible at low levels on the FlashGel™ System for RNA. FlashGel™ RNA Cassette run for 8 minutes at 225 V, followed by 20 minute hold prior to imaging. Lane 1: RNA marker (Lonza); Lane 2: 250 ng E. coli Total RNA; Lanes 3 – 7: E. coli Total RNA incubated with increasing levels of RNase A. Intact, denatured RNA shows sharp, clear bands on the FlashGel™ System. Partially degraded RNA has a smeared appearance, and completely degraded RNA appears as a low molecular weight smear.
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