For efficient transfection of primary human mesenchymal stem cells in the Nucleofector™ I/II/2b Device. The Nucleofector™ 2b Device (or older generations I or II) works cell type specific kits, each of them dedicated to an individual primary cell. This Nucleofector™ Kit is the optimal kit for efficient transfectiion of primary human mesenchymal stem cells (MSCs).
The respective cell type-specific Optimized Protocol or recommendation is available under ""Instructions"" or in our knowledge database. In this Optimized Protocol the best Nucleofection conditions are indicated. In addition, we share our experience and knowledge for treatment of this cell type.
The kit is available in different variants for either 10 or 25 or 4 x 25 reactions. Benefits: High transfection efficiencies for primary cells, Cells maintain functionality post transfection.
Applications : Transfection of cell numbers in the range of 2 × 105 to 2 × 107 cells , Low-throughput transfection in single cuvette format.
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