The X-VIVO™ 10 Media formulations are designed to support the generation of LAK cells in a serum-free environment. The original protocols involved the incubation of patient or normal donor peripheral blood lymphocytes (PBL) at 1.0 – 3.0 × 106 cells/ml for a period of 3 days in the presence of 1,000 Cetus units of rIL-2/ml. Optimal LAK cell generation is achieved when peri pheral blood lymphocytes are incubated for 3 – 10 days at a density of 1.0 – 6.0 × 106 cells/ml in the presence of 100 – 1,000 Cetus units of rIL-2.
All BioWhittaker™ Cell Culture Media products are for Research Use Only (RUO) and are not approved for human or veterinary use or for use in clinical or in vitro diagnostic procedures. If you require GMP grade media, contact Lonza for more details.
Superior media for most hematopoietic cells types including dendritic cells, lymphocytes, monocytes, macrophages and granulocytes.
Formulations only contain human derived components
More regulatory friendly as human component is replaced by a recombinant one
Highly cited in scientific literature
Clinical proven formulations providing process safety and constancy
High productivity resulting in a high viable cell yield
Serum Free media result in a more defined process and result in limited immune response
Efficient transgene expression Desirable T-cell phenotype
Proliferation of peripheral blood lymphocytes
Proliferation of tumor infiltrating lymphocytes
Cryopreservation of human tissue
Cultivation of human monocytes and macrophages
Cultivation of stem cells
Cultivation of dendritic cells
Cultivation of CAR-T cells
Endotoxin and Pyrogen Testing Products
The next chapter in endotoxin testing... Let's write it together.